estrogen receptor er positive breast cancer cell line  (ATCC)

Journal: Cells

Article Title: Identification of UDP-Glucuronosyltransferase 2B15 (UGT2B15) as a Target for IGF1 and Insulin Action

doi: 10.3390/cells11101627

Figure Lengend Snippet: Expression of UGT2B15 in breast cancer cell lines. ( A ) Total RNA was obtained from confluent T47D and MCF7 cells and UGT2B15 mRNA levels were measured by RT-QPCR. A value of 1 was assigned to the UGT2B15 mRNA levels in T47D cells. * p < 0.01 vs. T47D cells. ( B ) Total protein was obtained from confluent T47D and MCF7 cell lines and UGT2B15, IGF1R, INSR, and p53 protein levels were measured by Western blots. Relative protein levels are expressed as protein levels normalized to the corresponding HSP70 value. Results of a representative experiment are shown, repeated three times with similar results.

Article Snippet: The estrogen receptor (ER) positive breast cancer cell lines MCF7 and T47D were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cells

Article Title: Identification of UDP-Glucuronosyltransferase 2B15 (UGT2B15) as a Target for IGF1 and Insulin Action

doi: 10.3390/cells11101627

Figure Lengend Snippet: Regulation of UGT2B15 gene expression by IGF1 and insulin in breast cancer cell lines. MCF7 ( A ) and T47D ( B ) cells were treated with IGF1 or insulin (50 ng/mL) for 24 h, after which total RNA was prepared and UGT2B15 mRNA levels were measured by RT-QPCR. A value of 1 was given to the UGT2B15 mRNA levels in control, untreated cells. * p < 0.01 vs. respective control cells. ( C , D ) MCF7 and T47D cells were treated with IGF1 or insulin for 24 h, after which total protein was prepared and UGT2B15 and p53 levels were measured by Western blots. Bar graphs represent UGT2B15 levels normalized to the corresponding HSP70 values. A value of 100% was given to control, untreated cells.

Article Snippet: The estrogen receptor (ER) positive breast cancer cell lines MCF7 and T47D were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Gene Expression, Quantitative RT-PCR, Control, Western Blot

Journal: Cells

Article Title: Identification of UDP-Glucuronosyltransferase 2B15 (UGT2B15) as a Target for IGF1 and Insulin Action

doi: 10.3390/cells11101627

Figure Lengend Snippet: Effect of IGF1R and INSR inhibition on UGT2B15 gene expression. ( A ) MCF7 cells were treated with the selective IGF1R inhibitor AEW541 (10 mM) for 48 h (or left untreated, C), after which cells were harvested, total protein was prepared, and IGF1R, UGT2B15, and p53 levels were measured by Western blots. HSP70 levels were measured as a loading control. The bar graph denotes UGT2B15 and IGF1R levels in control (solid bars) and AEW541 treated cells (striped bars). ( B ) T47D cells were treated with the INSR inhibitor S961 (100 nM and 1 mM) for 2 h. Cells were then harvested and levels of phospho- and total-INSR, UGT2B15, and p53 levels were measured by Western blots. A value of 100% was given to control, untreated cells. * p < 0.01.

Article Snippet: The estrogen receptor (ER) positive breast cancer cell lines MCF7 and T47D were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Inhibition, Gene Expression, Western Blot, Control

Journal: Cells

Article Title: Identification of UDP-Glucuronosyltransferase 2B15 (UGT2B15) as a Target for IGF1 and Insulin Action

doi: 10.3390/cells11101627

Figure Lengend Snippet: Effect of UGT2B15 abrogation on IGF1R signaling and cellular proliferation. MCF7 ( A , B ) and T47D ( C , D ) were treated with siRNA against UGT2B15 (or NT for control purposes) for 72-h (MCF7) or 96 h (T47D). At the end of the incubation period, cells were harvested, and the levels of IGF1R, INSR, UGT2B15, phospho- and total- AKT and ERK1/2, and p53 were measured by Western blots. HSP70 levels were measured as a loading control. Relative protein levels are expressed as protein levels normalized to the corresponding HSP70 value. Results of a typical experiment are presented. For cell proliferation measurements, cells were treated with siRNA against UGT2B15 (or NT siRNA) for 72 h (MCF7) or 96 h (T47D). Cells were counted using a cell counter. A value of 100% was given to the cell number in NT-treated (control) cells. * p < 0.01 vs. NT-treated cells.

Article Snippet: The estrogen receptor (ER) positive breast cancer cell lines MCF7 and T47D were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control, Incubation, Western Blot

Journal: Developmental cell

Article Title: The bone microenvironment increases phenotypic plasticity of ER+ breast cancer cells

doi: 10.1016/j.devcel.2021.03.008

Figure Lengend Snippet: A. Experimental design of BoM lineage tracing using an inducible CRISPR-Cas9 hgRNA evolving barcoding system. iCas9-expressing MCF7 cells were stably transduced with homing guide RNA A21 (hgRNA-A21) before transplantation to bone and induced weekly for four weeks before LCM and targeted sequencing. We collected 19 lesions from femur (#1–12) and tibia (#13–19). Barcoded parental cells were labelled as #20.

Article Snippet: Human estrogen receptor positive (ER+) breast cancer cell lines MCF7, T47D, MDA-MB-361 and ZR75-30, pre-osteoblast cells hFOB-1.19, mesenchymal stem cells MSC, pre-osteoclast U937, and the mouse pre-osteoblast MC3T3-E1 were purchased from American Type Culture Collection (ATCC).

Techniques: CRISPR, Expressing, Stable Transfection, Transduction, Transplantation Assay, Sequencing

Journal: Developmental cell

Article Title: The bone microenvironment increases phenotypic plasticity of ER+ breast cancer cells

doi: 10.1016/j.devcel.2021.03.008

Figure Lengend Snippet: A. IF of BoMs showing ER expression in PDXs (HCI011 and WHIM9) and MCF7 cells, relatively to Receptor activator of nuclear factor-KB (RANK) expression in osteoclasts. Scale bars: 100μm. Dot plots show ER quantification in SCs. (n=3–5 lesions).

Article Snippet: Human estrogen receptor positive (ER+) breast cancer cell lines MCF7, T47D, MDA-MB-361 and ZR75-30, pre-osteoblast cells hFOB-1.19, mesenchymal stem cells MSC, pre-osteoclast U937, and the mouse pre-osteoblast MC3T3-E1 were purchased from American Type Culture Collection (ATCC).

Techniques: Expressing

Journal: Developmental cell

Article Title: The bone microenvironment increases phenotypic plasticity of ER+ breast cancer cells

doi: 10.1016/j.devcel.2021.03.008

Figure Lengend Snippet: A. Relative mRNA expression of ESR1 in 3D monoculture or co-culture of MCF7 with FOB. Data result from FACS-sorted MCF7. (n=3 technical replicates).

Article Snippet: Human estrogen receptor positive (ER+) breast cancer cell lines MCF7, T47D, MDA-MB-361 and ZR75-30, pre-osteoblast cells hFOB-1.19, mesenchymal stem cells MSC, pre-osteoclast U937, and the mouse pre-osteoblast MC3T3-E1 were purchased from American Type Culture Collection (ATCC).

Techniques: Expressing, Co-Culture Assay

Journal: Developmental cell

Article Title: The bone microenvironment increases phenotypic plasticity of ER+ breast cancer cells

doi: 10.1016/j.devcel.2021.03.008

Figure Lengend Snippet: A. Experimental summary diagram to evaluate molecular in cancer cells exposed to BME. TRAP-seq was performed on 3D co-culture of MCF7 and OGs (MSCs), RPPA on un-entrained (MCF7 and M7-SCP2) and bone-entrained cells (MCF7-Bo and M7-SCP2-Bo), and ATAC-seq on un-entrained (M7-SCP2) or bone-entrained (M7-SCP2-Bo) cells.

Article Snippet: Human estrogen receptor positive (ER+) breast cancer cell lines MCF7, T47D, MDA-MB-361 and ZR75-30, pre-osteoblast cells hFOB-1.19, mesenchymal stem cells MSC, pre-osteoclast U937, and the mouse pre-osteoblast MC3T3-E1 were purchased from American Type Culture Collection (ATCC).

Techniques: Co-Culture Assay

Journal: Developmental cell

Article Title: The bone microenvironment increases phenotypic plasticity of ER+ breast cancer cells

doi: 10.1016/j.devcel.2021.03.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human estrogen receptor positive (ER+) breast cancer cell lines MCF7, T47D, MDA-MB-361 and ZR75-30, pre-osteoblast cells hFOB-1.19, mesenchymal stem cells MSC, pre-osteoclast U937, and the mouse pre-osteoblast MC3T3-E1 were purchased from American Type Culture Collection (ATCC).

Techniques: Virus, Recombinant, Reverse Transcription, Transfection, Sequencing, Negative Control, Luciferase, Plasmid Preparation, Modification, Software